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Biology LibreTexts

3.10: Urea Broth

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  • Some bacteria are able to break urea into carbon dioxide and ammonia by means of the exoenzyme urease.


    1. Obtain a tube of urea broth.
    2. Using an inoculating loop, swish some of your assigned organism into the broth.
    3. Incubate the tube for at least 48 hours.
    4. After the incubation period, note the color of the broth.


    If urease is produced, the medium will be cerise.


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