12.3: Results

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A. ELISA test

Record the appearance of each of the wells here.

Well	Contents	Color appearance	Interpretation
1
2
3
4
5
6

B. Biolog

Record your Biolog results below.

Describe the colony that you picked for Biolog (color, etc.): _________________________________________ ________________________________________________________________

Use the sheet on the next page to record your results. In each box, write “+” if the well appears positive (purple color), “-” if there is no color change, and “+/-“ if the result is borderline (light purple).

Your instructor will show you how to enter your results into the database to achieve an identification.

An example of what a Biolog plate looks like is shown below.

Identification: ___________________________________

% match: _______________________________________

A1 Negative Control	A2 Dextrin	A3 D-Maltose	A4 D-Trehalose	A5 D-Cellobiose	A6 Gentiobiose	A7 Sucrose	A8 D-Turanose	A9 Stachyose	A10 Positive Control	A11 pH 6	A12 pH 5
B1 D-Raffinose	B2 α-D-Lactose	B3 D-Melibiose	B4 β-Methyl-D-Glucoside	B5 D-Salicin	B6 N-Acetyl-DGlucosamine	B7 N-Acetyl-β-D-Mannosamine	B8 N-Acetyl-D-Galactosamine	B9 N-Acetyl Neuraminic Acid	B10 1% NaCl	B11 4% NaCl	B12 8% NaCl
C1 α-D-Glucose	C2 D-Mannose	C3 D-Fructose	C4 D-Galactose	C5 3-Methyl Glucose	C6 D-Fucose	C7 L-Fucose	C8 L-Rhamnose	C9 Inosine	C10 1% Sodium Lactate	C11 Fusidic Acid	C12 D-Serine
D1 D-Sorbitol	D2 D-Mannitol	D3 D-Arabitol	D4 myo-Inositol	D5 Glycerol	D6 D-Glucose- 6- PO4	D7 D-Fructose- 6- PO4	D8 D-Aspartic Acid	D9 D-Serine	D10 Troleandomycin	D11 Rifamycin SV	D12 Minocycline
E1 Gelatin	E2 Glycyl-L-Proline	E3 L-Alanine	E4 L-Arginine	E5 L-Aspartic Acid	E6 L-Glutamic Acid	E7 L-Histidine	E8 L-Pyroglutamic Acid	E9 L-Serine	E10 Lincomycin	E11 Guanidine HCl	E12 Niaproof 4
F1 Pectin Acid	F2 D-Galacturonic Acid	F3 L-Galactonic Acid Lactone	F4 D-Gluconic Acid	F5 D-Glucuronic Acid	F6 Glucuronamide	F7 Mucic Acid	F8 Quinic Acid	F9 D-Saccharic Acid	F10 Vancomycin	F11 Tetrazolium Violet	F12 Tetrazolium Blue
G1 p-Hydroxy Phenylacetic Acid	G2 Methyl Pyruvate	G3 D-Lactic Acid Methyl Ester	G4 L-Lactic Acid	G5 Citric Acid	G6 α-KetoGlutaric Acid	G7 D-Malic Acid	G8 L-Malic Acid	G9 BromoSuccinic Acid	G10 Nalidixic Acid	G11 Lithium Chloride	G12 Potassium Tellurite
H1 Tween 40	H2 γ-aminobutyric Acid	H3 α- HydroxyButyric Acid	H4 β-HydroxyD,LButyric Acid	H5 α-Keto-Butyric Acid	H6 Acetoacetic Acid	H7 Propionic Acid	H8 Acetic Acid	H9 Formic Acid	H10 Aztreonam	H11 Sodium Butyrate	H12 Sodium Bromate