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12.3: Results

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    A. ELISA test

    Record the appearance of each of the wells here.

    Well Contents Color appearance Interpretation

    B. Biolog

    Record your Biolog results below.

    Describe the colony that you picked for Biolog (color, etc.): _________________________________________ ________________________________________________________________

    Use the sheet on the next page to record your results. In each box, write “+” if the well appears positive (purple color), “-” if there is no color change, and “+/-“ if the result is borderline (light purple).

    Your instructor will show you how to enter your results into the database to achieve an identification.

    An example of what a Biolog plate looks like is shown below.

    Identification: ___________________________________

    % match: _______________________________________

    Figure 12.3.1: Biolog plate
    A1 Negative Control A2 Dextrin A3 D-Maltose A4 D-Trehalose A5 D-Cellobiose A6 Gentiobiose A7 Sucrose A8 D-Turanose A9 Stachyose A10 Positive Control A11 pH 6 A12 pH 5
    B1 D-Raffinose B2 α-D-Lactose B3 D-Melibiose B4 β-Methyl-D-Glucoside B5 D-Salicin B6 N-Acetyl-DGlucosamine B7 N-Acetyl-β-D-Mannosamine B8 N-Acetyl-D-Galactosamine B9 N-Acetyl Neuraminic Acid B10 1% NaCl B11 4% NaCl B12 8% NaCl
    C1 α-D-Glucose C2 D-Mannose C3 D-Fructose C4 D-Galactose C5 3-Methyl Glucose C6 D-Fucose C7 L-Fucose C8 L-Rhamnose C9 Inosine C10 1% Sodium Lactate C11 Fusidic Acid C12 D-Serine
    D1 D-Sorbitol D2 D-Mannitol D3 D-Arabitol D4 myo-Inositol D5 Glycerol D6 D-Glucose- 6- PO4 D7 D-Fructose- 6- PO4 D8 D-Aspartic Acid D9 D-Serine D10 Troleandomycin D11 Rifamycin SV D12 Minocycline
    E1 Gelatin E2 Glycyl-L-Proline E3 L-Alanine E4 L-Arginine E5 L-Aspartic Acid E6 L-Glutamic Acid E7 L-Histidine E8 L-Pyroglutamic Acid E9 L-Serine E10 Lincomycin E11 Guanidine HCl E12 Niaproof 4
    F1 Pectin Acid F2 D-Galacturonic Acid F3 L-Galactonic Acid Lactone F4 D-Gluconic Acid F5 D-Glucuronic Acid F6 Glucuronamide F7 Mucic Acid F8 Quinic Acid F9 D-Saccharic Acid F10 Vancomycin F11 Tetrazolium Violet F12 Tetrazolium Blue
    G1 p-Hydroxy Phenylacetic Acid G2 Methyl Pyruvate G3 D-Lactic Acid Methyl Ester G4 L-Lactic Acid G5 Citric Acid G6 α-KetoGlutaric Acid G7 D-Malic Acid G8 L-Malic Acid G9 BromoSuccinic Acid G10 Nalidixic Acid G11 Lithium Chloride G12 Potassium Tellurite
    H1 Tween 40 H2 γ-aminobutyric Acid H3 α- HydroxyButyric Acid H4 β-HydroxyD,LButyric Acid H5 α-Keto-Butyric Acid H6 Acetoacetic Acid H7 Propionic Acid H8 Acetic Acid H9 Formic Acid H10 Aztreonam H11 Sodium Butyrate H12 Sodium Bromate

    12.3: Results is shared under a CC BY-NC-SA license and was authored, remixed, and/or curated by Joan Petersen & Susan McLaughlin.

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