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A. Throat cultures
1. Examine your blood agar plates and sketch your plate in the space provided, noting any specific characteristics of the colonies. In particular you should note the type of hemolysis you observe.
Did you observe colonies that showed α, β, and γ hemolysis? _______________________________________
Your instructor has demo plates of all types of hemolysis—be sure to view them if you do not see all types on your own plate.
B. Urine culture
1. Examine your blood agar plate, and record the type of hemolysis observed. What type(s) of hemolysis were observed on your BA plate?
Your sample: _____________________
Simulated sample: _________________
2. Examine your EMB plate, and note the color and appearance of the colonies you observe from your own urine sample as well as from the simulated sample. Describe the growth observed on the EMB plate for:
Your sample: ________________________________
Simulated sample: ___________________________
Sketch both plates (BA and EMB) in the space provided below.
Based on the appearance of the growth you observed, what can you conclude about the bacteria in your samples?
C. Anaerobic cultures
Your TSA plates were either incubated aerobically (on the tray) or anaerobically (within the anaerobic jar).
Before opening the jar, observe the indicator strip that was added when the jar was set up. Recall that the methylene blue strip appeared blue when it was first added to the jar. If anaerobic conditions were achieved within the jar, the indicator should have turned white.
What color is the indicator strip? __________________________________
Based on this, can you say proper conditions were achieved within the jar? _____________________________________________________________
For plates incubated aerobically: which bacteria shows growth on these plates? _______________________
For plates incubated anaerobically: which bacteria shows growth on these plates? _____________________
Based on your results, what can you conclude about these two organisms? ________________________________
Describe the type/amount of growth you observe on the plates on which the sowbug walked. ______________________________________________________________________________
Did the amount of microbes decrease from the first plate to the 4th? __________________________________________________________________________________________
Fill in the table on the next page. Use the reference materials available in the lab to fill in the information for each organism (your instructor may assign you to research a particular species or group).
|Vector name||Common name of vector||Name of pathogen transmitted by vector||Characteristics of pathogen (type of organism, morphology)||Name of disease||Body part affected||Additional information|