- To examine the function and properties of enzymes in living cells
- To distinguish between substrate, enzyme, and product in a reaction
- To collect and analyze data on tissue-specific catalase activity
- To collect and analyze data on the effect of temperature on catalase action
Thumbnail: Enzyme changes shape by induced fit upon substrate binding to form enzyme-substrate complex. Hexokinase has a large induced fit motion that closes over the substrates adenosine triphosphate and xylose. Binding sites in blue, substrates in black and Mg2+ cofactor in yellow. (PDB: 2E2N, 2E2Q). Image used with permission (CC BY 4.0l Thomas Shafee).