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Biology LibreTexts

10.5: Concept Review

  • Page ID
    24189
    1. What is the basis for the separation of DNA fragments using electrophoresis?
    2. What is the charge of the samples?
    3. What do you think would happen in the molecules had the opposite charge?
    4. What would happen if you attached to red and black electrode on the wrong sides?
    5. If a DNA molecule has three restriction sites, for restriction enzyme A, how many fragments would be produced.
    6. Using restriction enzymes a piece of DNA is cut into 4 pieces, the fragments are separated using gel electrophoresis. Below are the results:
      10.4restrictionResults.PNG
      1. Which fragment would be the largest?
      2. Which fragment would be the smallest?
    7. Below are one strand of two samples of DNA.
      1. Sample #1: C A G T G A T C T C G A A T T C G C T A G T A A C G T T
      2. Sample #2: T C A T G A A T T C C T G G A A T C A G C A A A T G C A
      3. Both samples are treated with a restriction enzyme restriction enzyme EcoR1 which cuts DNA at the sequence GAATTC.
      4. Indicate the number of fragments that would be generated by each sample.
    8. Compare the DNA fingerprint from the crime scene to the 3 suspects. Which suspect committed the crime?
    10.4crimeComparison.PNG

    This work is in the public domain - http://www.genome.gov/glossary/