3.5: APPENDIX C2 Primer design worksheet

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Elizabeth Vogel Taylor
Massechusetts Institute of Technology via MIT OpenCourseWare

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Name:__________________________________________________________________

Group members: __________________________________________________________

Section/TA name_________________________________________________________

Point mutation (use numbering from Appendix B2)______________________________

Please also circle your desired mutation below:
(688)tcc cccaactacg acaagtggga gatggaacgc
721 acggacatca ccatgaagca caagctgggc gggggccagt acggggaggt gtacgagggc
781 gtgtggaaga aatacagcct gacggtggcc gtgaagacct tgaaggagga caccatggag
841 gtggaagagt tcttgaaaga agctgcagtc atgaaagaga tcaaacaccc taacctggtg
901 cagctccttg gggtctgcac ccgggagccc ccgttctata tcatcactga gttcatgacc
961 tacgggaacc tcctggacta cctgagggag tgcaaccggc aggaggtgaa cgccgtggtg
1021 ctgctgtaca tggccactca gatctcgtca gccatggagt acctggagaa gaaaaacttc
1081 atccacagag atcttgctgc ccgaaactgc ctggtagggg agaaccactt ggtgaaggta
1141 gctgattttg gcctgagcag gttgatgaca ggggacacct acacagccca tgctggagcc
1201 aagttcccca tcaaatggac tgcacccgag agcctggcct acaacaagtt ctccatcaag
1261 tccgacgtct gggcatttgg agtattgctt tgggaaattg ctacctatgg catgtcccct
1321 tacccgggaa ttgacctgtc ccaggtgtat gagctgctag agaaggacta ccgcatggag
1381 cgcccagaag gctgcccaga gaaggtctat gaactcatgc gagcatgttg gcagtggaat
1441 ccctctgacc ggccctcctt tgctgaaatc caccaagcct ttgaaacaat gttccaggaa
1501 tccagtatct cagacgaagt ggaaaaggag ctgggg

Corresponding amino acid substitution (use numbering from Appendix B1)___________

Forward Primer (5’ to 3’)
________________________________________________________________________

Reverse Primer (5’ to 3’)
________________________________________________________________________

Percent GC _______________ Tm_______________________________