8.2: Procedure
- Page ID
- 49706
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Place an Elodea sprig in a test tube that is equipped with a rubber stopper and a bent glass tube. Before capping the tube, fill the tube with water. Add enough so that when the stopper is inserted into the tube, the solution comes to rest at about one-fourth the length of the bent glass tubing.
Figure \(\PageIndex{1}\) Formation of O\(_2\) bubbles displaces the water and moves the edge to the left in this diagram. Consumption of O\(_2\) makes the edge move to the right in this picture. The distance moved should be proportional to the amount of O\(_2\) produced or consumed.
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Place the tube near a light source on the lab bench.
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Record the position of the solution “edge.” As soon as the edge of the solution in the thin glass tube begins to move away from the Elodea, time the reaction for 10 minutes.
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At the end of the 10 minutes, record the new position of the edge of the solution in the tube. Record in millimeters (mm) how far the edge moved.
Note: always pay attention to the direction that the fluid is moving. Moving in opposite directions must mean that something is either being consumed or produced. Be sure to record direction of movement with “\(+\)” or “\(-\)”.
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Move tube away from under the lamp, and wait 10 min to introduce break between “day” and “night”. Meanwhile, you might answer some questions from the report page.
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Wrap the test tube in aluminum foil. Wait 10 minutes and measure the distance the edge recedes. This is usually the negative number.
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Return the Elodea and clean and dry the equipment and lab area.