5.4: Procedure

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Using the directions provided to you by your instructor, prepare three slides—one with onion skin (epidermis), one with an Elodea leaf, and one with human cheek cells:

Slide 1.

Using a drop of IKI (iodine in potassium iodide) prepare a wet mount slide of onion skin from the bulb leaf. The sample must be extremely thin! Observe the cells at different magnifications.

Slide 2.

Using a drop of water, prepare a wet mount of an Elodea leaf.

Slide 3.

Prepare a slide of your own cheek cells stained with methylene blue.
Cover your slide with the cover glass, touch the surface with one side first, then slowly release (instructor will show how).
Place your slide inside manipulator, locate the smallest lens (\(5\times\)) over the object on a distance about 3 cm, and looking into the microscope, simultaneously adjust the coarse knob.
When your object is in focus, switch to bigger lenses. When using bigger lenses, it is recommended to look on the object, simultaneously rotating the fine knob to and fro.
Using the approach discussed above, estimate the size (maximal length) of each kind of cells.