3.36: Bacterial Susceptibility to Antibiotics (Kirby-Bauer Test)
- Page ID
- 91012
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Preparation
- TSA petri plates (enough for 3 per group)
- 18-24 hour TSB cultures of Staphylococcus aureus (one per group or enough for groups to share)
Materials
- TSA petri plates (enough for 3 per group)
- 18-24 hour TSB cultures of Staphylococcus aureus (one per group or enough for groups to share)
- test tube racks (1 per group)
- Bunsen burners (1 per group)
- strikers (1 per group)
- sterile cotton swabs (3 per group)
- waste containers with disinfectant for used cotton swabs (1 per group)
- tweezers (1 per antibiotic type - label tweezers so they do not get mixed up)
- antibiotic disks (one of each type per group):
- erythromycin (15 µg)
- penicillin G (10 units)
- streptomycin (10 µg)
- tetracycline (30 µg)