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3.17: Bacterial Oxygen Requirements

  • Page ID
    90994
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    Preparation

    • 24 hour cultures of Pseudomonas aeruginosa, Escherichia coli, and a Clostridium species of choice (if using) - Clostridium species are obligate anaerobes so cultivation will require strict anaerobic conditions (enough for groups to share)
    • thioglycollate tubes (enough for each group to have 2-3, depending on the number of species being tested)
    • TSA plates (2 per group)

    Materials

    • 24 hour cultures of Pseudomonas aeruginosa, Escherichia coli, and a Clostridium species of choice (if using) - Clostridium species are obligate anaerobes so cultivation will require strict anaerobic conditions (enough for groups to share)
    • thioglycollate tubes (enough for each group to have 2-3, depending on the number of species being tested)
    • labeling tape (1 per group)
    • labeling markers (1 per group)
    • test tube racks (1 per group)
    • inoculating loops (1 per group)
    • strikers (1 per group)
    • Bunsen burners (1 per group)
    • TSA plates (2 per group)
    • anaerobic jar or bags with heat sealer
    • GasPak anaerobic generators
    • anaerobic indicators

    This page titled 3.17: Bacterial Oxygen Requirements is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Rosanna Hartline.

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