Skip to main content
Biology LibreTexts

11.4: Laboratory Activities and Assignment

  1. Last updated
  2. Save as PDF
  • Page ID
    53680

    • \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)

    Laboratory Activities and Assignment

    Part 1: Review of Introduction to Skeletal Muscles

    1. Label the figure showing the structure of a skeletal muscle below with the following terms:

    • epimysium
    • perimysium
    • endomysium
    • fascicle
    • muscle fiber

    Diagram of skeletal muscle for labeling

    2. Below is a microscopic image of a longitudinal view of muscle fibers magnified by 1000x. Label the following structures on the image:

    • muscle fiber
    • striations
    • nuclei
    • A band
    • I band
    • H zone
    • Z disc (challenge)

    Microscopic image of skeletal muscle fibers

    3. Below is a microscopic image of a cross section of muscle fibers magnified by 200x. Label the following structures on the image:

    • muscle fiber
    • nuclei
    • endomysium
    • fascicle
    • perimysium

    Microscopic image of skeletal muscle fascicle cross section

    4. Label the figure showing examples of skeletal muscles below with the following terms:

    • origin
    • insertion
    • extensor
    • flexor

    Diagram of skeletal muscle for labeling

    Part 2: Introduction to Skeletal Muscles Laboratory Activities

    1. Examine a slide of skeletal muscle viewing muscle fibers with a longitudinal view. Illustrate the tissue and label the following structures. Indicate the total magnification of the view you illustrate in the space provided.

    • muscle fiber
    • striations
    • nuclei
    • A band
    • I band
    • H zone (challenge)
    • Z disc (challenge)

    Location for sample illustration.

    2. Examine a slide of skeletal muscle that has been cross-sectioned. Illustrate the tissue and label the following structures. Indicate the total magnification of the view you illustrate in the space provided.

    • epimysium
    • fascicle
    • perimysium
    • muscle fiber
    • endomysium
    • nuclei

    Location for sample illustration.

    3. Below is electron micrograph (very high magnification - 15,000x magnification in this image) of a longitudinal view of multiple myofibrils (structures found inside of a single muscle fiber) stacked top to bottom and their component sarcomeres lined up (left to right). Work with your group to label the following structures:

    • myofibril
    • sarcomere
    • dark band of striation
    • light band of striation
    • M line
    • H zone
    • A band
    • I band
    • Z disc