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6.III: Protein sequencing.

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  • A. Sanger's reagent, FDNB (fluorodinitrobenzene), modifies N-terminus for determination via amino acid analysis.

    B. Edman degradation. Uses PITC (phenylisothiocyanate) to sequentially remove N-terminal amino acids for analysis.

    PITC can be used to sequentially remove and analyze amino acids from a polypeptide on solid support.

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