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6.III: Protein sequencing.

  • Page ID
    7271
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    A. Sanger's reagent, FDNB (fluorodinitrobenzene), modifies N-terminus for determination via amino acid analysis.

    770px-Sanger_peptide_end-group_analysis.svg.png

    B. Edman degradation. Uses PITC (phenylisothiocyanate) to sequentially remove N-terminal amino acids for analysis.

    Phenyl_isothiocyanate.svg.png
    PITC can be used to sequentially remove and analyze amino acids from a polypeptide on solid support.

    edmandegradation.png


    6.III: Protein sequencing. is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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