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Biology LibreTexts

6.II: Amino acid separation.

  • Page ID
  • A. Acid hydrolysis at high temperature and pressure (6 M HCl, 121°C, 15 atmospheres, 4 - 5 hr).

    (note: also hydrolyses side chain peptide/amide bonds in asparagine and glutamine to produce aspartate and glutamate)

    B. Ion exchange chromatography. (external link: here is one schematic animation of the method)

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