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Biology LibreTexts

12: Detecting Genes and Gene Products

  • Page ID
    • 12.1: DNA Analysis- Blotting and Hybridization
      Bands of DNA in an electrophoretic gel form only if most of the DNA molecules are of the same size, such as following a PCR reaction, or restriction digestion of a plasmid. In other situations, such as after restriction digestion of chromosomal (genomic) DNA, there will be a large number of variable size fragments in the digest and it will appear as a continuous smear of DNA, rather than distinct bands.

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