6.3.4: Materials and Procedures
Materials
-
Organisms-
- Staphylococcus epidermidis
- Escherichia coli
- Lactobacillus acidophilus
- 1 plate of each Sodium Chloride (\(\ce{NaCl}\)) concentration:
- TSA with 2.0% \(\ce{NaCl}\)
- TSA with 5.0% \(\ce{NaCl}\)
- TSA with 10.0%\(\ce{NaCl}\)
Procedures
Inoculate each plate with the following organisms: Staphylococcus saprophyticus, Escherichia coli, Lactococcus lactis
- Use a single streak on ⅓ of the plate for each organism; make sure to label each streak line with the correct organism:
Staphylococcus saprophyticus, E. coli, Lactococcus lactis → TSA with 2.0% \(\ce{NaCl}\)
Staphylococcus saprophyticus, E. coli, Lactococcus lactis → TSA with 5.0% \(\ce{NaCl}\)
Staphylococcus saprophyticus, E. coli, Lactococcus lactis → TSA with 10.0% \(\ce{NaCl}\)
- Invert and incubate.
Results
- After incubation assess the growth of each bacteria on each plate.
- Fill out the chart below:
Enter your ratings for each bacterium/salinity and give the salinity classification for bacterium.
|
Organism |
2.0% \(\ce{NaCl}\) |
5.0% \(\ce{NaCl}\) |
10.0% \(\ce{NaCl}\)l |
Optimum salinity |
Salinity Classification |
|---|---|---|---|---|---|