6.2.4: Materials and Procedures
Materials
-
Organisms used from the Student Stock Cultures(TSB):
- Proteus vulgaris
- Escherichia coli
- Alcaligenes faecalis
- Lactobacillus acidophilus
- 4 tubes: TSB pH 4.0
- 4 tubes: TSB pH 6.0
- 4 tubes: TSB pH 8.0
- 4 tubes: TSB pH 10.0
Procedures
Each Group :
-
Use a sterile transfer pipette to inoculate each of the 4 organisms into each pH tube:
- add 1 drop of broth culture to each pH broth tube.
- Staphylococcus saprophyticus → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
- Escherichia coli → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
- Alcaligenes viscolactis → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
- Lactococcus lactis → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
- Place the tubes in buckets and incubate in your classroom incubator.
Results
Enter your ratings for each bacterium/pH and give the pH classification for bacterium.
|
Organism |
pH 4 |
pH 6 |
pH 8 |
pH 10 |
Optimum pH |
pH Classification |
|---|---|---|---|---|---|---|