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7.4: Results

  • Page ID
    52257
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    Record your results for the metabolic tests in the tables on the following pages. 

    A. Carbohydrate Fermentation

    Observe the results of your own carbohydrate fermentation test, as well as the tests done by your table partners. You can compare your inoculated tubes with the negative controls in the front of the class at the instructor’s table. Record the results in the table below.

    The following convention is used when noting the results of fermentation experiments.

    A = acid

    G = gas

    AG means that both acid and gas are present

    If neither acid nor gas is present, you can write “negative”

    Bacteria Glucose Lactose Sucrose
    Proteus vulgaris      
    Escherichia coli      
    Bacillus subtilis      
    Streptococcus faecalis      

    B. TSI

    Bacterium Glucose Lactose Sucrose Gas production H2S production
               
               
               
               

     

    C. Casein Hydrolysis

    Observe your casein plate. It is helpful to hold it up to the light so you can detect the clear zones. Make a drawing of your results the circle below. Indicate the location of the bacterial growth and draw any clear zones that are present. Record your results in the table below.

    plate2.png

    Bacteria Clear zone? Result (+/-)
    A. Enterobacter aerogenes    
    B. Bacillus subtilis    
    C. Negative control    

     

     

    D. Catalase Activity

    Add a dropper full of H2O2 to the surface of the slant. Record your results below.

    Bacteria Presence of bubbles Result (+/-)
    Bacillus megaterium    
    Unknown    

    E. Oxidase

    Bacteria Color Oxidase Result (+/-)
    Bacillus megaterium    
    Pseudomonas fluorescens    
         
         

     

     

    F. SIM Tubes

    NOTE:   Be sure to look on the surface of the agar as well as within the deep stab for evidence of motility.

    A.       SIM agar deep tubes

     

    Culture

    Motility

    Sulfide

    Indole

    Klebsiella aerogenes

     

     

     

    Staphylococcus epidermidis

     

     

     

    Proteus vulgaris

     

     

     

    Unknown

     

     

     

     

     

     


    This page titled 7.4: Results is shared under a CC BY-NC-SA license and was authored, remixed, and/or curated by Joan Petersen & Susan McLaughlin.