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6.7: Results

  • Page ID
    52346
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     Plaque Assay

     Count the number of plaques 

    Look at all of your plates prepared last period. Record the number of plaques following the same procedure used in counting bacterial colonies . Calculate the PFU/mL of the original phage sample. Remember, your plated 100 µL (0.1 mL) of the phage dilutions.

     

    1. Record data from your phage plates here:

     

     

    Total dilution

    Plaque Counts

    10-4

     

    10-5

     

    10-6

     

            10-7

     

    PFU/mL of original phage sample:______________________

     

     

      2.         Record phage enumeration data for the class here:

    Student pairs

    Dilution with 30-300 plaques

    Number of plaques on plate

    PFU/mL in original T4 phage sample.

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    13

     

     

     

                                                                                    Class Average:

     

    Testing Oxygen Requirements

    Illustrate the growth pattern of each bacterial species inoculated into the tube of thioglycollate agar using the pictures below. Record any other distinguishing characteristics of the growth of each species. Name the classification of the organism based on its oxygen requirements, aerobe, anaerobe, of facultative, in the space below.

    clipboard_e63ea7c60da709b3d0e521f7ee9e991e3.png clipboard_e14f45939d241c548d7bbb00ac2831ea1.pngclipboard_eb8b454cada129526dbc895b4c51fc1f6.png

     


    6.7: Results is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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