5.4: Results

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Enumeration and Dilution

1. Read lab procedure section on how to obtain colony counts and CFU/mL in the original Serratia marcescens sample.

2. Record data from your Serratia marcescens plates here:

final dilution	colony count
10^-5
10^-6
10^-7
10^-8
10^-9

CFU/mL of original Serratia marcescens sample =____________________

3. Record the class data here for the Serratia marcescens broth culture:

Individual Student data	Dilution with 30-300 colonies	Number of colonies on plate	*CFU/mL in original Serratia marcescens* sample**
1
2
3
4
5
6
7
8
9
10
11
12

Class average:_____________________

4. Practice question

Final sample dilution	Colony Count*	CFU/mL in original sample
1/1000	135
10^-6	48
10^-8		6.5 x 10⁹
	99	9.9 x l0⁶

*Remember the 30-300 colonies per plate rule.