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6.9: Glossary- The Cellular Level of Organization

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    active transport: form of transport across the cell membrane that requires input of cellular energy

    amphipathic: describes a molecule that exhibits a difference in polarity between its two ends, resulting in a difference in water solubility

    anaphase: third stage of mitosis (and meiosis), during which sister chromatids separate into two new nuclear regions of a dividing cell

    anticodon: consecutive sequence of three nucleotides on a tRNA molecule that is complementary to a specific codon on an mRNA molecule

    autolysis: breakdown of cells by their own enzymatic action

    autophagy: lysosomal breakdown of a cell’s own components

    cell cycle: life cycle of a single cell, from its birth until its division into two new daughter cells

    cell membrane: membrane surrounding all animal cells, composed of a lipid bilayer interspersed with various molecules; also known as plasma membrane

    centriole: small, self-replicating organelle that provides the origin for microtubule growth and moves DNA during cell division

    centromere: region of attachment for two sister chromatids

    centrosome: cellular structure that organizes microtubules during cell division

    channel protein: membrane-spanning protein that has an inner pore which allows the passage of one or more substances

    checkpoint: progress point in the cell cycle during which certain conditions must be met in order for the cell to proceed to a subsequence phase

    chromatin: substance consisting of DNA and associated proteins

    chromosome: condensed version of chromatin

    cilia: small appendage on certain cells formed by microtubules and modified for movement of materials across the cellular surface

    cleavage furrow: contractile ring that forms around a cell during cytokinesis that pinches the cell into two halves

    codon: consecutive sequence of three nucleotides on an mRNA molecule that corresponds to a specific amino acid

    concentration gradient: difference in the concentration of a substance between two regions

    cyclin-dependent kinase (CDK): one of a group of enzymes associated with cyclins that help them perform their functions

    cyclin: one of a group of proteins that function in the progression of the cell cycle

    cytokinesis: final stage in cell division, where the cytoplasm divides to form two separate daughter cells

    cytoplasm: internal material between the cell membrane and nucleus of a cell, mainly consisting of a water-based fluid called cytosol, within which are all the other organelles and cellular solute and suspended materials

    cytoskeleton: “skeleton” of a cell; formed by rod-like proteins that support the cell’s shape and provide, among other functions, locomotive abilities

    cytosol: clear, semi-fluid medium of the cytoplasm, made up mostly of water

    DNA polymerase: enzyme that functions in adding new nucleotides to a growing strand of DNA during DNA replication

    DNA replication: process of duplicating a molecule of DNA

    diffusion: movement of a substance from an area of higher concentration to one of lower concentration

    diploid: condition marked by the presence of a double complement of genetic material (two sets of chromosomes, one set inherited from each of two parents)

    electrical gradient: difference in the electrical charge (potential) between two regions

    endocytosis: import of material into the cell by formation of a membrane-bound vesicle

    endoplasmic reticulum (ER): cellular organelle that consists of interconnected membrane-bound tubules, which may or may not be associated with ribosomes (rough type or smooth type, respectively)

    exocytosis: export of a substance out of a cell by formation of a membrane-bound vesicle

    exon: one of the coding regions of an mRNA molecule that remain after splicing

    extracellular fluid (ECF): fluid exterior to cells; includes the interstitial fluid, blood plasma, and fluid found in other reservoirs in the body

    facilitated diffusion: diffusion of a substance with the aid of a membrane protein

    flagellum: appendage on certain cells formed by microtubules and modified for movement

    G0 phase: phase of the cell cycle, usually entered from the G1 phase; characterized by long or permanent periods where the cell does not move forward into the DNA synthesis phase

    G1 phase: first phase of the cell cycle, after a new cell is born

    G2 phase: third phase of the cell cycle, after the DNA synthesis phase

    Golgi apparatus: cellular organelle formed by a series of flattened, membrane-bound sacs that functions in protein modification, tagging, packaging, and transport

    gene expression: active interpretation of the information coded in a gene to produce a functional gene product

    gene: functional length of DNA that provides the genetic information necessary to build a protein

    genome: entire complement of an organism’s DNA; found within virtually every cell

    glycocalyx: coating of sugar molecules that surrounds the cell membrane

    glycoprotein: protein that has one or more carbohydrates attached

    helicase: enzyme that functions to separate the two DNA strands of a double helix during DNA replication

    histone: family of proteins that associate with DNA in the nucleus to form chromatin

    homologous: describes two copies of the same chromosome (not identical), one inherited from each parent

    hydrophilic: describes a substance or structure attracted to water

    hydrophobic: describes a substance or structure repelled by water

    hypertonic: describes a solution concentration that is higher than a reference concentration

    hypotonic: describes a solution concentration that is lower than a reference concentration

    integral protein: membrane-associated protein that spans the entire width of the lipid bilayer

    intermediate filament: type of cytoskeletal filament made of keratin, characterized by an intermediate thickness, and playing a role in resisting cellular tension

    interphase: entire life cycle of a cell, excluding mitosis

    interstitial fluid (IF): fluid in the small spaces between cells not contained within blood vessels

    intracellular fluid (ICF): fluid in the cytosol of cells

    intron: non-coding regions of a pre-mRNA transcript that may be removed during splicing

    isotonic: describes a solution concentration that is the same as a reference concentration

    kinetochore: region of a centromere where microtubules attach to a pair of sister chromatids

    ligand: molecule that binds with specificity to a specific receptor molecule

    lysosome: membrane-bound cellular organelle originating from the Golgi apparatus and containing digestive enzymes

    messenger RNA (mRNA): nucleotide molecule that serves as an intermediate in the genetic code between DNA and protein

    metaphase plate: linear alignment of sister chromatids in the center of the cell, which takes place during metaphase

    metaphase: second stage of mitosis (and meiosis), characterized by the linear alignment of sister chromatids in the center of the cell

    microfilament: the thinnest of the cytoskeletal filaments; composed of actin subunits that function in muscle contraction and cellular structural support

    microtubule: the thickest of the cytoskeletal filaments, composed of tubulin subunits that function in cellular movement and structural support

    mitochondrion: one of the cellular organelles bound by a double lipid bilayer that function primarily in the production of cellular energy (ATP)

    mitosis: division of genetic material, during which the cell nucleus breaks down and two new, fully functional, nuclei are formed

    mitotic phase: phase of the cell cycle in which a cell undergoes mitosis

    mitotic spindle: network of microtubules, originating from centrioles, that arranges and pulls apart chromosomes during mitosis

    multipotent: describes the condition of being able to differentiate into different types of cells within a given cell lineage or small number of lineages, such as a red blood cell or white blood cell

    mutation: change in the nucleotide sequence in a gene within a cell’s DNA

    nuclear envelope: membrane that surrounds the nucleus; consisting of a double lipid-bilayer

    nuclear pore: one of the small, protein-lined openings found scattered throughout the nuclear envelope

    nucleolus: small region of the nucleus that functions in ribosome synthesis

    nucleosome: unit of chromatin consisting of a DNA strand wrapped around histone proteins

    nucleus: cell’s central organelle; contains the cell’s DNA

    oligopotent: describes the condition of being more specialized than multipotency; the condition of being able to differentiate into one of a few possible cell types

    organelle: any of several different types of membrane-enclosed specialized structures in the cell that perform specific functions for the cell

    osmosis: diffusion of molecules down their concentration across a selectively permeable membrane

    passive transport: form of transport across the cell membrane that does not require input of cellular energy

    peripheral protein: membrane-associated protein that does not span the width of the lipid bilayer, but is attached peripherally to integral proteins, membrane lipids, or other components of the membrane

    peroxisome: membrane-bound organelle that contains enzymes primarily responsible for detoxifying harmful substances

    phagocytosis: endocytosis of large particles

    pinocytosis: endocytosis of fluid

    pluripotent: describes the condition of being able to differentiate into a large variety of cell types

    polypeptide: chain of amino acids linked by peptide bonds

    polyribosome: simultaneous translation of a single mRNA transcript by multiple ribosomes

    promoter: region of DNA that signals transcription to begin at that site within the gene

    prophase: first stage of mitosis (and meiosis), characterized by breakdown of the nuclear envelope and condensing of the chromatin to form chromosomes

    proteome: full complement of proteins produced by a cell (determined by the cell’s specific gene expression)

    RNA polymerase: enzyme that unwinds DNA and then adds new nucleotides to a growing strand of RNA for the transcription phase of protein synthesis

    reactive oxygen species (ROS): a group of extremely reactive peroxides and oxygen-containing radicals that may contribute to cellular damage

    receptor-mediated endocytosis: endocytosis of ligands attached to membrane-bound receptors

    receptor: protein molecule that contains a binding site for another specific molecule (called a ligand)

    ribosomal RNA (rRNA): RNA that makes up the subunits of a ribosome

    ribosome: cellular organelle that functions in protein synthesis

    S phase: stage of the cell cycle during which DNA replication occurs

    selective permeability: feature of any barrier that allows certain substances to cross but excludes others

    sister chromatid: one of a pair of identical chromosomes, formed during DNA replication

    sodium-potassium pump: (also, Na+/K+ ATP-ase) membrane-embedded protein pump that uses ATP to move Na+ out of a cell and K+ into the cell

    somatic cell: all cells of the body excluding gamete cells

    spliceosome: complex of enzymes that serves to splice out the introns of a pre-mRNA transcript

    splicing: the process of modifying a pre-mRNA transcript by removing certain, typically non-coding, regions

    stem cell: cell that is oligo-, multi-, or pleuripotent that has the ability to produce additional stem cells rather than becoming further specialized

    telophase: final stage of mitosis (and meiosis), preceding cytokinesis, characterized by the formation of two new daughter nuclei

    totipotent: embryonic cells that have the ability to differentiate into any type of cell and organ in the body

    transcription factor: one of the proteins that regulate the transcription of genes

    transcription: process of producing an mRNA molecule that is complementary to a particular gene of DNA

    transfer RNA (tRNA): molecules of RNA that serve to bring amino acids to a growing polypeptide strand and properly place them into the sequence

    translation: process of producing a protein from the nucleotide sequence code of an mRNA transcript

    triplet: consecutive sequence of three nucleotides on a DNA molecule that, when transcribed into an mRNA codon, corresponds to a particular amino acid

    unipotent: describes the condition of being committed to a single specialized cell type

    vesicle: membrane-bound structure that contains materials within or outside of the cell

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