9.10: SIM Deep Test Procedure
SIM Deep Test Procedure
Name: _____________________________________________________
Course Section: ______________________________________________
The following activity will teach students how to inoculate a SIM deep test. Post-inoculation, students will analyze growth patterns to access sulfur reduction, indole production, and motility.
| Materials |
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| Procedure |
| Day 1 |
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Step 1:
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| Day 2 |
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Step 2:
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Results
In the table below, record your observations for colonies grown SIM media.
| Species | Medium color | Hydrogen sulfide production (+/-) | Kovac's reagent color | Indole (+/-) | Location of growth (thin / thick) | Motility (+/-) |
|---|---|---|---|---|---|---|
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Escherichia coli |
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Proteus mirabilis |
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Enterobacter aerogenes |
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General Questions
1. A bacterial species that is positive for hydrogen sulfide production could be producing H 2 S in one of two ways. What are these? Explain your answer.
2. Is hydrogen sulfide black? Explain your answer.
3. A bacterial species that is positive for indole produces what enzyme? What does this enzyme do?
4. What is the role of Kovac's reagent in the indole test?
5. A bacterial species that is positive for motility has what type of bacterial appendage? How can one tell if motility has occurred within the SIM media?
6. What are the primary symptoms of Salmonella infection, and which populations are most at risk for severe complications?
7. How does the SIM test aid in the diagnosis of gastrointestinal pathogens?