12.2: Lab 12 Procedures
- Page ID
- 159738
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)PROCEDURE 1: FUNGAL GROWTH
Equipment we’ll need:
- Plates from last week
- Review the answers to the design questions in the answer sheet from last class.
- Obtain the group’s plates. Do not open the plates.
- Examine the plates carefully looking for even minor differences in the colonies growing on the plate.
- Record the results in the Answer Sheet.
Clean up/Disposal
- Dispose of agar plates into the red biohazard bin.
PROCEDURE 2: PLANT SCAVENGER HUNT
- Fill in Table 3 in the Answer Sheet to compare the different plant groups using the information from above.
- With Table 3 complete, walk about our building and campus to find representative species of each major plant group. It is possible to find all seven groups from Table 3.
- Option for inclement weather or evening classes: using your favorite search engine look for images of our campus and identify plants from those images. Get creative with ways to explore these plant groups and where they might grow.
- Take a picture of each plant group you identify and bring them back to the classroom to share.
PROCEDURE 3: PLANT, LEAF, & FLOWER ANATOMY SAMPLES
Part 1: Leaf Structures - Stomata
Equipment we’ll need:
- Clean microscope slides
- Boxwood leaf
- Cover slips
- Black marker
- Obtain a clean slide and coverslip.
- Remove a small Boxwood leaf. Color in the backside of the leaf with a black marker.
- This helps us view the stomata.
- Place the specimen on the slide with the backside of the leaf facing up. Cover with a coverslip.
- Using the scanning (4x) objective lens, get the specimen into focus. Once in focus, move the objective to low power (10x). Draw what is seen in the Answer Sheet.
Part 2: Flower Structures
Equipment we’ll need:
- One flower
- Dissecting tray
- Scissors
- Scalpel/Razor blade
- Needle
- Obtain one dissecting tray, any necessary tools, and a flower specimen.
- Observe the flower and try to identify the parts using the image above.
- Remove the sepals and stem being careful not to harm the base of the flower.
- Remove all petals being careful not to harm the inner reproductive organs.
- Identify and remove the stamen(s) from the flower. If possible, remove the anther from the filament. Another option is to rub the anther on the paper to see the dusty pollen grains.
- Carefully using a scalpel or razor blade, cut the ovary directly in half to see the ovules.
- Place or draw each flower part in the Answer Sheet.
Clean up/Disposal
Microscope
- Remove slide from microscope.
- Lower the microscope stage all the way down.
- Put the 4x objective lens in place over the stage.
- Turn off the microscope.
- Unplug the microscope and wrap the cord neatly around the base.
- Cover the microscope and return to its appropriate cabinet. Then lock the cabinet and return the key.
Slides
- Remove the coverslip from the wet mounts. Dispose of the coverslip in the trash.
- Dispose of the boxwood piece in the trash.
- Rinse the slide clean. Set slides out to air dry on the towels.