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27.3: Materials and Procedures

  • Page ID
    40330
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    Materials

    • Gloves
    • Hardy StaphTEXBlue Kit:
      • Reagents
      • Wooden Sticks
      • Latex Agglutination Cards
    • Disposable inoculating loops
    • Even and Odd numbered tubes of unknown bacterial cultures
    • StaphTEX product information sheet

    Procedures

    1. Shake well, and then place a drop of the LATEX REAGENT in each of the first 4 reaction circles on the card.
    2. Add 1 small drop of (+) control to the first circle. Mix into the Latex Reagent to the edges of the circle with the wooden stick.
    3. Add 1 drop of the (-) control reagent to the second circle. Mix with the wooden stick.
    4. Add one loop of growth (2 small colonies) from an EVEN numbered unknown culture to the third circle. Mix with the wooden stick.
    5. Add one loop of growth (2 small colonies) from an ODD numbered unknown culture to the fourth circle. Mix with the wooden stick.
    6. Gently rock the card back and forth for 20 seconds and read the reaction immediately. A positive reaction will have visible clumps in the mixture—it looks like “blue pepper.” A negative test will be a uniform suspension.
    7. Compare the unknowns to the controls to determine if an unknown is S. aureus.

    Results

    1. Draw your reaction here, label each circle with the reagents/cultures mixed in each:
    clipboard_ee398b858da89d05a748c723002ed360f.png
    1. Record your results:

    Unknown tube #

    Agglutination Reaction (+ or -)

    Positive ID of S. aureus ? (+ or -)

    ID according to the instructor key: (name of organism)

    Contributors and Attributions


    This page titled 27.3: Materials and Procedures is shared under a CC BY license and was authored, remixed, and/or curated by Kelly C. Burke.

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