27.3: Materials and Procedures
- Page ID
- 40330
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- Gloves
- Hardy StaphTEXBlue Kit:
- Reagents
- Wooden Sticks
- Latex Agglutination Cards
- Disposable inoculating loops
- Even and Odd numbered tubes of unknown bacterial cultures
- StaphTEX product information sheet
Procedures
- Shake well, and then place a drop of the LATEX REAGENT in each of the first 4 reaction circles on the card.
- Add 1 small drop of (+) control to the first circle. Mix into the Latex Reagent to the edges of the circle with the wooden stick.
- Add 1 drop of the (-) control reagent to the second circle. Mix with the wooden stick.
- Add one loop of growth (2 small colonies) from an EVEN numbered unknown culture to the third circle. Mix with the wooden stick.
- Add one loop of growth (2 small colonies) from an ODD numbered unknown culture to the fourth circle. Mix with the wooden stick.
- Gently rock the card back and forth for 20 seconds and read the reaction immediately. A positive reaction will have visible clumps in the mixture—it looks like “blue pepper.” A negative test will be a uniform suspension.
- Compare the unknowns to the controls to determine if an unknown is S. aureus.
Results
- Draw your reaction here, label each circle with the reagents/cultures mixed in each:
- Record your results:
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Unknown tube # |
Agglutination Reaction (+ or -) |
Positive ID of S. aureus ? (+ or -) |
ID according to the instructor key: (name of organism) |
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Contributors and Attributions
Kelly C. Burke (College of the Canyons)