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12.5: Post-lab Questions

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    1. A buffer is a chemical often added to growth media to prevent shifts in the pH of the media. A buffering system will absorb or release \(\ce {H+}\) ions to prevent the media from becoming too acidic or alkaline, respectively. Why would it be important to add a buffer to growth media?
    2. Based on your results, is there overlap in the pH ranges of the organisms? Why might this be?
    3. The pH of seawater is around 8.3. What factors or media adjustments would you have to make when trying to culture marine organisms?

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