6.4: Materials and Procedures
- Page ID
- 40186
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Materials
Wet Mount Materials:
- Glass slides
- Coverslips
Live Material:
- Turbatrix aceti- Vinegar Eels (Nematoda)
Preserved Materials:
- Flukes and Tapeworms
- Dissected Ascaris (Unsegmented Roundworms)
- Prepared Slides:
- Phylum Platyhelminthes: (on back bench)
- Class Trematoda (Flukes)
- Fasciola hepatica (liver flukes)
- Clonorchis sinensis (Oriental Liver Fluke)
- Schistosoma sp. (Blood flukes)
- Class Cestoda (segmented Flatworms)
- Taenia sp. (proglotids) (Tapeworm)
- Dypilidium caninum (Dog tapeworm)
- Phylum Nematoda (unsegmented Roundworms):
- Ascaris lumbricoides
- Enterobius vermicularis (Pinworm)
- Necator americanus (Hookworm)
- Trichinella spiralis
Procedures
- Wet Mount of Turbatrix aceti (Nematoda):
- Place one drop of sample onto a cleaned slide. Add a drop of Detain if desired to slow down the movement of the nematode.
- Carefully place a coverslip over sample.
- Observe under the microscope (low and high power) with bright-field, dark-field, and phase contrast. Draw some of your observations.
- Turbatrix can also be observed via a dissecting scope.
- Prepared specimens: Observe and draw.
- Prepared slides: Observe, draw, label, and measure the helminths and their structures. Pay close attention to the label on each slide. The slide will give hints as to what you will observe. Terms/abbreviations that you may see on the label:
WM=Whole Mount-the entire organism is on the slide
CS=Cross Section-the organism has been cut across the body in the vertical plane and is mounted on end. You are looking through a cross section of the organism. Think of making orange slices and looking through the cut segments.
TS=Transverse Section
LS=Longitudinal Section-cuts across the horizontal plane.
Results
Contributors and Attributions
Kelly C. Burke (College of the Canyons)