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Biology LibreTexts

6.4: Materials and Procedures

  • Page ID
    40186
  • Materials

    Wet Mount Materials:

    • Glass slides
    • Coverslips

    Live Material:

    • Turbatrix aceti- Vinegar Eels (Nematoda)

    Preserved Materials:

    • Flukes and Tapeworms
    • Dissected Ascaris (Unsegmented Roundworms)
    • Prepared Slides:
    • Phylum Platyhelminthes: (on back bench)
    • Class Trematoda (Flukes)
    • Fasciola hepatica (liver flukes)
    • Clonorchis sinensis (Oriental Liver Fluke)
    • Schistosoma sp. (Blood flukes)
    • Class Cestoda (segmented Flatworms)
    • Taenia sp. (proglotids) (Tapeworm)
    • Dypilidium caninum (Dog tapeworm)
    • Phylum Nematoda (unsegmented Roundworms):
    • Ascaris lumbricoides
    • Enterobius vermicularis (Pinworm)
    • Necator americanus (Hookworm)
    • Trichinella spiralis

    Procedures

    1. Wet Mount of Turbatrix aceti (Nematoda):
      1. Place one drop of sample onto a cleaned slide. Add a drop of Detain if desired to slow down the movement of the nematode.
      2. Carefully place a coverslip over sample.
      3. Observe under the microscope (low and high power) with bright-field, dark-field, and phase contrast. Draw some of your observations.
      4. Turbatrix can also be observed via a dissecting scope.
    2. Prepared specimens: Observe and draw.
    3. Prepared slides: Observe, draw, label, and measure the helminths and their structures. Pay close attention to the label on each slide. The slide will give hints as to what you will observe. Terms/abbreviations that you may see on the label:

    WM=Whole Mount-the entire organism is on the slide

    CS=Cross Section-the organism has been cut across the body in the vertical plane and is mounted on end. You are looking through a cross section of the organism. Think of making orange slices and looking through the cut segments.

    TS=Transverse Section

    LS=Longitudinal Section-cuts across the horizontal plane.

    Results

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    Contributors and Attributions

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