1.12: Streaking for Isolation Assignment
Name ______________________
| Sample source |
Area (1, 2 or 3) where isolated colonies are found |
Number of different looking colonies |
Is there evidence of contamination? |
Your T-streak Evaluation
isolated colonies at least three
streak pattern: correct order, correct direction
amount crossovers (not too little, not too much)
entire surface used
no gouging of the agar
no inappropriate crossovers
90 o angle of streaks
adequate room for final area
growth pattern: a lot to a little
label : all components, legible, on bottom of plate
incubated upside-down
Post Lab Questions
- What streaking technique is used here?
- A gross lab sink is sampled with a cotton swab and plated onto this TSA plate. What streaking technique is used here?
Was the technique a good choice? Why or why not?
- A pure culture is streaked onto this TSA plate. What technique is used here?
Was the technique a good choice?
What did the person likely do wrong when performing the technique? (There are at least 2 things.)
- What technique is used on this plate?
Was the technique a good choice?
What did the person likely do wrong when performing the technique?