11.5: Concept Review
- What is the basis for the separation of DNA fragments using electrophoresis?
- What is the charge of the samples?
- What do you think would happen in the molecules had the opposite charge?
- What would happen if you attached to red and black electrode on the wrong sides?
- If a DNA molecule has three restriction sites, for restriction enzyme A, how many fragments would be produced.
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Using restriction enzymes a piece of DNA is cut into 4 pieces, the fragments are separated using gel electrophoresis. Below are the results:
- Which fragment would be the largest?
- Which fragment would be the smallest?
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Below are one strand of two samples of DNA.
- Sample #1: C A G T G A T C T C G A A T T C G C T A G T A A C G T T
- Sample #2: T C A T G A A T T C C T G G A A T C A G C A A A T G C A
- Both samples are treated with a restriction enzyme restriction enzyme EcoR1 which cuts DNA at the sequence GAATTC.
- Indicate the number of fragments that would be generated by each sample.
- Compare the DNA fingerprint from the crime scene to the 3 suspects. Which suspect committed the crime?
This work is in the public domain - http://www.genome.gov/glossary/