On or Off? On or Off? On or Off?
That is the key question. Gene regulation in eukaryotes is a highly regulated process usually involving many proteins, which either bind to each other or bind to the DNA.
Eukaryotic Gene Regulation
In eukaryotic cells, the start of transcription is one of the most complicated parts of gene regulation. There may be many regulatory proteins and regulatory elements involved. Regulation may also involve enhancers. Enhancers are distant regions of DNA that can loop back to interact with a gene’s promoter.
The TATA Box
Different types of cells have unique patterns of regulatory elements that result in only the necessary genes being transcribed. That’s why a skin cell and nerve cell, for example, are so different from each other. However, some patterns of regulatory elements are common to all genes, regardless of the cells in which they occur. An example is the TATA box, so named because it has a core sequence of TATAAA. This is a regulatory element that is part of the promoter of most eukaryotic genes. A number of regulatory proteins bind to the TATA box, forming a multi-protein complex. It is only when all of the appropriate proteins are bound to the TATA box that RNA polymerase recognizes the complex and binds to the promoter. Once RNA polymerase binds, transcription begins. To see a video showing the role of the TATA box in the initiation of transcription, go to this link: http://www.youtube.com/watch?v=6tqPsI-9aQA.
Regulation During Development
The regulation of gene expression is extremely important during the development of an organism. Regulatory proteins must turn on certain genes in particular cells at just the right time so the organism develops normal organs and organ systems. Homeobox genes are an example of genes that regulate development. They code for regulatory proteins that switch on whole series of major developmental genes. In insects, homeobox genes called hox genes ensure that body parts such as limbs develop in the correct place. Figure below shows how a mutation in a hox gene can affect an insect’s development. Other organisms, including humans, also have how genes. You can learn more about homeobox genes at this link:http://www.youtube.com/watch?v=LFG-aLidT8s.
Effect of Hox Gene Mutation. Scientists caused a mutation in a hox gene of this fruit fly. As a result of the mutation, a leg grew out of its head where an antenna should have developed.
Gene Expression and Cancer
The mutations that cause cancer generally occur in two types of regulatory genes: tumor-suppressor genes and proto-oncogenes (see Figure below). These genes produce regulatory proteins that control the cell cycle. When the genes mutate, cells with mutations divide rapidly and without limits, potentially resulting in a tumor and cancer.
How Cancer Develops. This flow chart shows how a series of mutations in tumor-suppressor genes and proto-oncogenes leads to cancer.
- Regulation of transcription in eukaryotes is generally more complex than in prokaryotes. It involves unique regulatory elements in different cells as well as common regulatory elements such as the TATA box.
- Regulation is especially important during development. It may involve regulatory genes such as homeobox genes that switch other regulatory genes on or off.
- Mutations in regulatory genes that normally control the cell cycle cause cancer.
Use this resource to answer the questions that follow.
- Master genes control basic body plans at www.dnalc.org/resources/nobel/lewis_nauulein_wieschaus.html.
- How were genes that control early embryonic development identified?
- Describe the polarity of the fertilized egg, as it relates to protein expression.
- What does "segmentation" refer to in the developing fly?
- What is a gap mutant?
- Describe the antennapedia mutant.
- What is a homeobox protein?
- Identify the TATA box and its function in transcription.
- What is a homeobox gene?
- Why is gene regulation especially important during development?
- What are tumor-suppressor genes and proto-oncogenes?
- Sketch how an insect with a mutated hox gene might look. Explain your sketch.