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8.4: Genetic linkage and Genetic Maps

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    Gregor Mendel analyzed the pattern of inheritance of seven pairs of contrasting traits in the domestic pea plant. He did this by cross-breeding dihybrids; that is, plants that were heterozygous for the alleles controlling two different traits.

    Producing dihybrids (F1)

    He mated a variety that was pure-breeding (hence homozygous) for round (RR), yellow (YY) seeds with one that was pure-breeding for wrinkled (rr), green (yy) seeds. All the offspring (F1) produced from this mating were dihybrids; that is, heterozygous for each pair of alleles (RrYy). Furthermore, all the seeds were round and yellow, showing that the genes for round and yellow are dominant.

    Mating the dihybrids to produce an F2 generation

    Figure 8.4.1 Dihybrid Cross

    Mendel then crossed these dihybrids. If it is inevitable that round seeds must always be yellow and wrinkled seeds must be green, then he would have expected that this would produce a typical monohybrid cross: 75% round-yellow; 25% wrinkled-green. But, in fact, his mating generated seeds that showed all possible combinations of the color and texture traits.

    • 9/16 of the offspring were round-yellow
    • 3/16 were round-green
    • 3/16 were wrinkled-yellow, and
    • 1/16 were wrinkled-green

    Rule of Independent Assortment

    Finding in every case that each of his seven traits was inherited independently of the others, he formed his "second rule" the Rule of Independent Assortment:

    The inheritance of one pair of factors (genes) is independent of the inheritance of the other pair.

    Today we know that this rule holds only if two conditions are met:

    • the genes are on separate chromosomes or
    • the genes are widely separated on the same chromosome.

    Mendel was lucky in that every pair of genes he studied met one requirement or the other. The table shows the chromosome assignments of the seven pairs of alleles that Mendel studied. Although all of these genes showed independent assortment, several were, in fact, syntenic with three loci occurring on chromosome 4 and two on chromosome 1. However, the distance separating the syntenic loci was sufficiently great that the genes were inherited as though they were on separate chromosomes.

    Trait Phenotype Alleles Chromosome
    Seed form round-wrinkled R-r 7
    Seed color yellow-green I-i 1
    Pod color green-yellow Gp-gp 5
    Pod texture smooth-wrinkled V-v 4
    Flower color purple-white A-a 1
    Flower location axial-terminal Fa-fa 4
    Plant height tall-dwarf Le-le 4

    With the rebirth of genetics in the 20th century, it quickly became apparent that Mendel's second rule does not apply to many matings of dihybrids. In many cases, two alleles inherited from one parent show a strong tendency to stay together as do those from the other parent. This phenomenon is called linkage.

    An example of linkage

    Figure 8.4.2. Linkage in corn

    Start with two different strains of corn (maize).

    • one that is homozygous for two traits
      • yellow kernels (C,C) which are filled with endosperm causing the kernels to be
      • smooth (Sh,Sh).
    • a second that is homozygous for
      • colorless kernels (c,c) that are wrinkled because their endosperm is
      • shrunken (sh,sh)

    When the pollen of the first strain is dusted on the silks of the second (or vice versa), the kernels produced (F1) are all yellow and smooth. So the alleles for yellow color (C) and smoothness (Sh) are dominant over those for colorlessness (c) and shrunken endosperm (sh).

    To simplify the analysis, mate the dihybrid with a homozygous recessive strain (ccshsh). Such a mating is called a test cross because it exposes the genotype of all the gametes of the strain being evaluated.

    According to Mendel's second rule, the genes determining color of the endosperm should be inherited independently of the genes determining texture. The F1 should thus produce gametes in approximately equal numbers.

    • CSh, as inherited from one parent.
    • csh, as inherited from the other parent
    • Csh, a recombinant
    • cSh, the other recombinant.

    All the gametes produced by the doubly homozygous recessives would be csh.

    If the inheritance of these genes observes Mendel's second rule; i.e., shows independent assortment, union of these gametes should produce approximately equal numbers of the four phenotypes. But as the chart shows, there is instead a strong tendency for the parental alleles to stay together. It occurs because the two loci are relatively close together on the same chromosome. Only 3.0% of the gametes contain a recombinant chromosome.

    Figure 8.4.3 Recombination

    During prophase I of meiosis, pairs of duplicated homologous chromosomes unite in synapsis and then nonsister chromatids exchange segments during crossing over. It is crossing over that produces the recombinant gametes. In this case, whenever a crossover occurs between the locus for kernel color and that for kernel texture, the original combination of alleles (CSh and csh) is broken up and a chromosome containing Csh and one containing cSh will be produced.

    Chromosome Maps

    The percentage of recombinants formed by F1 individuals can range from a fraction of 1% up to the 50% always seen with gene loci on separate chromosomes (independent assortment). The higher the percentage of recombinants for a pair of traits, the greater the distance separating the two loci. In fact, the percent of recombinants is arbitrarily chosen as the distance in centimorgans (cM), named for the pioneering geneticist Thomas Hunt Morgan. In our case, the c and sh loci are said to be 3.0 cM apart. The procedure can be continued with another locus on the same chromosome.


    Test crossing a corn plant that is dihybrid for the C,c alleles and the alleles for bronze color (Bz, bz) produces 4.6% recombinants. So these two loci are 4.6 cM apart. However, is the bz locus on the same side of c as sh or is it on the other side?

    The answer can be found by test crossing the dihybrid Shsh, Bzbz. If the percentage of recombinants is less than 4.6%, then bz must be on the same side of locus c as locus sh. If greater than 4.6%, it must be on the other side.

    Figure 8.4.4 Plot Linkage

    In fact, the recombination frequency is 2.0%, telling us that the actual order of loci is

    Mapping by linkage analysis is best done with loci that are relatively close together; that is, within a few centimorgans of each other. Why? Because as the distance between two loci increases, the probability of a second crossover occurring between them also increases.

    But a second crossover would undo the effect of the first and restore the parental combination of alleles. These would show up as nonrecombinants. Thus as the distance between two loci increases, the percentage of recombinants that forms understates the actual distance in centimorgans that separates them. And, in fact, that has happened in this example. Using a three-point cross reveals the existence of a small number of double recombinants and tells us that the actual distance c—bz is indeed 5 cM as we would expect by summing

    • c—sh = 3 cM
    • sh—bz = 2 cM

    and not the 4.6 cM revealed by the dihybrid cross.

    A three-point cross also tells us the gene order in a single cross rather than the three we needed here.

    There are other problems with preparing genetic maps of chromosomes.

    • The probability of a crossover is not uniform along the entire length of the chromosome.
      • Crossing over is inhibited in some regions (e.g., near the centromere).
      • Some regions are "hot spots" for recombination (for reasons that are not clear). Approximately 80% of genetic recombination in humans is confined to just one-quarter of our genome.
    • In humans, the frequency of recombination of loci on most chromosomes is higher in females than in males. Therefore, genetic maps of female chromosomes are longer than those for males.

    Figure 8.4.5 Genetic Map of Chromosome 9

    A genetic map of chromosome 9 (the one that carries the C, Sh, and bz loci) of the corn plant (Zea mays) is shown above. If one maps in small intervals from one end of a chromosome to the other, the total number of centimorgans often exceeds 100 (as you can see for chromosome 9). However, even for widely-separated loci, the maximum frequency of recombinants that can form is 50%. And this is also the frequency of recombinants that we see for genes independently assorting on separate chromosomes. So we cannot tell by simply counting recombinants whether a pair of gene loci is located far apart on the same chromosome or are on different chromosomes. As we saw above, several of Mendel's independently assorting traits are controlled by genes on the same chromosome but located so far apart that they are inherited as if they were located on different chromosomes.

    Genes that are present on the same chromosome are called syntenic.

    What about humans?

    Obviously one cannot perform controlled matings of humans to map our chromosomes. However, it is possible to estimate map positions by examining linkage in several generations of relatives.

    For example, blood samples from several large Mormon families in Utah, where all the members of at least three generations were alive to be sampled, have been collected and stored. These have already been used to establish genetic linkage relationships and will be available in the years ahead to study other human genes as they are identified.

    Genetic versus Physical Maps

    Chromosome mapping by counting recombinant phenotypes produces a genetic map of the chromosome. But all the genes on the chromosome are incorporated in a single molecule of DNA. Genes are simply portions of the molecule (open reading frames or ORFs) encoding products that create the observed trait (phenotype). The rapid progress in DNA sequencing has produced complete genomes for hundreds of microbes and several eukaryotes.

    Having the complete sequence makes it possible to determine directly the order and spacing of the genes. Maps drawn in this way are called physical maps.

    What is the relationship between the genetic map and the physical map of a chromosome? As a very rough rule of thumb, 1 cM on a chromosome encompasses 1 megabase (1 Mb = 106 bp) of DNA. But for the reasons mentioned above, this relationship is only approximate. Although the genetic maps of human females average 90% longer than the same maps in males, their chromosomes contain the same number of base pairs. So their physical maps are identical.