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6.2: Structural analysis of pulse-labeled DNA molecules

  • Page ID
    338
  • Figure 6.3 Restriction map of SV40

    Table 6.1. Appearance of radiolabel into restriction fragments of completed SV40 DNA molecules. The relative amount of pulse label from each restriction fragment is given below (the relative amount of pulse label is the 3H/32P ratio of each fragment, corrected for thymidine content and normalized to 1 for fragment A).

    Relative amount of pulse label

    Fragment

    5 min

    10 min

    15 min

    A

    1.0

    1.0

    1.0

    B

    3.9

    3.0

    2.3

    C

    0

    0.75

    0.75

    D

    0.92

    0.86

    1.1

    E

    1.8

    2.0

    1.7

    F

    4.0

    3.1

    2.4

    G

    5.4

    4.2

    2.6

    H

    1.7

    2.5

    2.0

    I

    2.7

    3.0

    2.2

    J

    4.9

    3.7

    2.6

    K

    2.4

    2.9

    1.9

    Figure 6.4.B.

    Question 6.2: What would the pattern be for unidirectional replication?

    Question 6.3: What would be the pattern if there were two origins, say in fragments E and H, with bidirectional replication from each?

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