11.E: Genomics and Systems Biology (Exercises)

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These are homework exercises to accompany Nickle and Barrette-Ng's "Online Open Genetics" TextMap. Genetics is the scientific study of heredity and the variation of inherited characteristics. It includes the study of genes, themselves, how they function, interact, and produce the visible and measurable characteristics we see in individuals and populations of species as they change from one generation to the next, over time, and in different environments.

Study Questions

11.1 What are the advantages of high-throughput –omics techniques compared to studying a single gene or protein at a time? What are the disadvantages

11.2 What would the chromatogram from a capillary sequencer look like if you accidentally added only template, primers, polymerase, and fluorescent terminators to the sequencing reaction?

11.3 What are the advantages and disadvantages of clone-by-clone vs. whole genome shotgun sequencing?

11.4 How could you use DNA sequencing to identify new species of marine microorganisms?

11.5 Explain how you could use a microarray to identify wheat genes that have altered expression during drought?

11.6 A microarray identified 100 genes whose transcripts are abundant in tumors, but absent in normal tissues. Do any or all of these transcripts cause cancer? Explain your answer.

11.7 How could you ensure that each spot printed on a microarray contains DNA for only one gene?

11.8 What would the spots look like on a microarray after hybridization, if each spot contained a random mixture of genes?

11.9 What would the spots look like if the hybridization of green and red labeled DNA was done at low stringency?