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Biology LibreTexts

8: Agarose gel electrophoresis

  • Page ID
    17544
  • Screen Shot 2019-01-03 at 10.50.21 PM.png

    Agarose gels are used to analyze DNA molecules. These gels are simple to construct, because they rely only on
    the gelling properties of agarose. Molecules are separated by size and visualized with fluorescent intercalating
    dyes. In this lab, you will analyze the products of the PCR reactions from the previous lab.


    Objectives

    At the end of this lab, students should be able to:

    • Prepare an agarose gel for separating DNA molecules.

    • Separate DNA molecules by electrophoresis.

    • Visualize DNA molecules on agarose gels using intercalating dyes.

    • Estimate the approximate sizes of DNA molecules using size standards.