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7.5: Exercise 3 - Design yeast colony PCR

  • Page ID
    17535
  • In this lab, each team will be able to perform six PCR reactions to identify your three S. cerevisiae strains. You will have the option of using any of the MET primers listed above, as well as the KANR primer B. It will not be possible to test every strain with both the GSPA-GSPB and GSPA-KANR B combinations. Use your results from the selective plating experiment as you work with your team to devise a strategy that will allow you to positively identify your met strains.

    List the primer pairs that you will use for the reactions, together with the predicted sizes of the PCR products in your notebook.