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11.6: Test yourself

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  • The pRS426 plasmid is 3419 bp long and has restriction sites recognized by AvaII at posi- tions 1514, 1736 and 2683 in its sequence. Draw a map of pRS426 below, showing the positions of the AvaII restriction sites. (Be sure to show the position of nucleotide 1 in the sequence.)

    Next to the drawing, list the sizes of the restriction fragments that would be generated by digest- ing pRS426 with AvaII.