At the end of this laboratory, students will be able to:
- select and adjust the most suitable micropipetteto transfer a given volume.
- accurately transfer microliter volumes.
- use a spectrophotometer to measure light absorbance.
- explain how experimental errors affect measurements.
Welcome to the microworld! In this class, you will be working with microorganisms. These include yeast and bacteria, millions of which would fit into a period on this page. You will also be working with costly reagents, such as plasmids and enzymes. Therefore, in every experiment, you will be required to accurately measure volumes as small as a few microliters(μL). Micropipetteswill allow you to do this accurately and precisely.