3.4: Part II- Pipetting Practice
- Page ID
- 138900
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A. Microplate Art
Materials
- p20 pipette (1)
- p200 micropipette (1)
- P-20/P-200 tips
- Microplate art set (design cards, colored dyes, and 96-well microplate) (1)
- Analytical or electronic balance
Procedure
- Obtain a 96-well microplate, a design card, and tubes of colored dyes.
- Write the Microplate Art Design number in your lab notebook.
- Using the gram balance, obtain the weight of your 96 well microplate and record in your notebook.
- Using the p200 micropipette with tip, dispense 50 µl of dye into the wells written on the design card.
- Once you have finished pipetting, weigh your completed microplate, and record in your lab notebook.
Results
- Be sure to record your weight in grams of your microplate pre/post pipetting in your lab notebook.
- Using these values, calculate your percent error of the microplate you just created. Include the calculation in your lab notebook.
- Take a picture of your microplate design and include this in your lab notebook.
Conclusion
- Was your percent error below +/- 5%? If your percent error was above this range, elaborate on the potential causes.
- Did your pattern look correct? How could you avoid errors in the future?
B. Micropipette Practice Matrix
Materials
- p20 pipette (1)
- p200 micropipette (1)
- 1.5ml microfuge tubes (3)
- Permanent marker
- Analytical or electronic balance
Procedure
- Label three microfuge tubes: 1, 2, 3,
- Weigh each tube before placing any liquid inside.
- Draw table 3.1 in your lab notebook and use it to record your data.
Results
|
Tube # |
Weight of |
Weight of |
Theoretical weight of dye |
Actual |
% Error |
|---|---|---|---|---|---|
|
1 |
|||||
|
2 |
|||||
|
3 |
- Deliver the volumes indicated in Table 3.2 into each of the 3 labeled tubes.
|
Tube # |
Micropipette |
Red Dye (µl) |
Blue Dye (µl) |
Green Dye (µl) |
|---|---|---|---|---|
|
1 |
P1000 |
210 |
435 |
332 |
|
2 |
P200 |
110 |
153 |
67 |
|
3 |
P20 |
15 |
17 |
10 |
- Weigh each tube after pipetting.
- Determine the theoretical weight of the dye using the information about the weight of a mL of the dye solution at room temperature provided by your instructor.
- Determine the % error for each tube.
Conclusion
Based on your data comment on the following in your lab notebook:
- Which micropipette gave the most precise measurement?
- Which micropipette gave the most accurate measurement?
- What may have contributed to higher percent errors?