Skip to main content
Biology LibreTexts

9E: Metabolic Control Analysis and Systems Biology

  • Page ID
    4614
  • \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)

    Learning Objectives

    • describe the steady state in context of an isolated single enzyme-catalyzed reaction and for the enzyme when part of a complex metabolic pathway in vivo;
    • state and describe what is required to perform a metabolic control analysis on a biological pathway;
    • write ordinary differential equations to describe the change in concentration of analytes in a given set of coupled reactions;
    • write mass balance expressions for Eo and use it to calculate the fraction of each enzyme form assuming rapid equilibrium;
    • draw "wire" diagrams showing activation and inhibition for coupled reactions;
    • define the flux control coefficient verbally and in the form of a mathematic equation, and explain how to obtain it graphically;
    • use flux control coefficients to explain how control of flux in a pathway is distributed through all enzyme-catalyzed reactions in that pathway;
    • analyze the result of a flux control analysis of a pathway like glycolysis;
    • describe why small changes in the enzymes that have the greatest ΔG values in glycolysis have minor effects in the metabolic flux in glycolysis;
    • define the concentration control coefficient verbally and in the form of a mathematic equation
    • analyze the result of a concentration control analysis of a pathway like glycolysis;
    • define the elasticity coefficient verbally and in the form form of a mathematic equation, and explain how to obtain it graphically
    • state whether the different metabolic control coefficients are global properties of the system or local properties of a given enzyme


    9E: Metabolic Control Analysis and Systems Biology is shared under a CC BY-NC-SA license and was authored, remixed, and/or curated by Henry Jakubowski.

    • Was this article helpful?