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Biology LibreTexts

2B: Composition, Sequence and Conformational Analysis of Proteins

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  • Learning Objectives

    describe in general terms the procedures and chemical steps in the determinations of the following for proteins:
    • molecular weight
    • presence of certain specific amino acids
    • amino acid composition
    • N and C terminal amino acid
    • specific amino acid necessary for binding and activity
    • amino acid sequence
    • secondary structure
    • 3D structure

    Thumbnail: Structure of human hemoglobin. The proteins α and β subunits are in red and blue, and the iron-containing heme groups in green. From PDB: 1GZX​. (GNU; Proteopedia Hemoglobin).

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