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4.10: Lineweaver-Burk Plots

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  • Figure 4.9.1: Line-Weaver Burk Plot

    For a Lineweaver-Burk, the manipulation is using the reciprocal of the values of both the velocity and the substrate concentration. The inverted values are then plotted on a graph as \(1/V\) vs. \(1/[S\)]. Because of these inversions, Lineweaver-Burk plots are commonly referred to as ‘double-reciprocal’ plots. As can be seen at left, the value of \(K_M\) on a Lineweaver Burk plot is easily determined as the negative reciprocal of the x-intercept , whereas the \(V_{max}\) is the inverse of the y-intercept. Other related manipulation of kinetic data include Eadie-Hofstee diagrams, which plot V vs V/[S] and give \(V_{max}\) as the Y-axis intercept with the slope of the line being \(-K_M\).