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Lab 13: Isolation and Identification of Enterobacteriaceae and Pseudomonas, Part 2

  • Page ID
    3452
  • Overview

    The concept behind the case studies presented in Lab 12 used to illustrate the Enterobacteriaceae and Pseudomonas is for you and your lab partners as a group to:

    1. First come up with a valid diagnosis of the general type of infectious disease seen in your case study and then identify the bacterium causing that infection, and

    2. Support your group’s diagnose based on:

    a. Any relevant facts in the patient’s history. (A reliable on-line source will be used to support this.)
    b. The patient’s signs and symptoms. (A reliable on-line source will be used to support this.)
    c. Each of the individual lab tests given in your case study.
    d. All microbiological lab tests you performed as part of the project.

    The due date for this report can be found on the class calendar. Remember, you are working as a group to solve a problem. Your grade for this lab is based on the completeness of your report and written evidence of the critical thinking process that went into making and supporting your diagnosis, therefore, it is critical that all members of the group participate, question any conclusions being made by the group, and contribute to the report. Remember, you are trying to convince your instructor that you understand how the diagnosis was made by supporting that diagnosis with data. Your group will work together to write the report and submit one hard copy of that report for your group. Part of your grade will be based on evaluation of your work by your team members.

    Each member of the group must:

    1. Print a copy of each of the two rubrics from the links above.

    2. Print and fill out a copy of the Team Member Evaluation Form from the link above.

    3. Staple them together and hand them in to me the day your Lab 12 Case Study Lab Report is due.

    Be sure to handle all the bacterial cultures you are using in lab today as if they are pathogens! Be sure to wash and sanitize your hands well at the completion of today’s lab.

    Also, make sure you observe several MacConkey agar plates and the Cetrimide agar plate used by others in your lab so that you can answer practical questions from Lab 12 and Lab 13. The Performance Objectives for Lab 13 tell you what you are expected to be able to do on the practical.

    Case Study Lab Report for Labs 12 and 13:
    The Enterobacteriaceae and Pseudomonas

    Each member of the group must:

    1. Print a copy of each of the two rubrics from the links above.

    2. Print and fill out a copy of the Team Member Evaluation Form from the link above.

    3. Staple them together and hand them in to me the day your Lab 12 Case Study Lab Report is due.

    For more information on writing your Lab Report, see Course evaluation for Lab (Core labs, case studies, lab quizzes) under Course Info in the menu of the BIOL 230 website.

    Click here to print a fresh Word document copy of this lab report.

    Your Name:

    Others in your group:

    Unknown number (1-8):

    Lab section:

    Date:

    Case Study #1A from Lab 12

    A 66 year old female with a history of recurring urinary tract infections and multiple antibiotic therapies presents with frequency and urgency of urination, dysuria, suprapubic discomfort, lower back pain, and a temperature of 99.2°F. A complete blood count (CBC) shows leukocytosis with a left shift. A urine dipstick shows a positive leukocyte esterase test, a positive nitrite test, 30mg of protein per deciliter, and red blood cells in the urine.

    Case Study #1B from Lab 12

    72 year old female who is diabetic and a smoker was admitted to the hospital with a leg wound that is not healing. She appears confused and anxious, has a temperature of 102°F, a heart rate of 101 beats per minute, a respiration rate of 29 breaths per minute, a blood pressure of 94/32 mm Hg, a urine output of only 110 cc for the last 8 hours, and a total white blood cell count of 2300/µL. A blood culture is taken.

    Contributors and Attributions

    • Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)