1. Distinguish between the Ziehl-Neelson and Kinyoun methods of acid-fast staining.
2. Why is it important to leave the malachite green on the slide for at least 10 minutes in the endospore staining procedure?
3. Do you think you would find more endospores in a freshly prepared culture or in an older culture of Bacillus subtilis? Explain.
4. Why do you think an infection caused by an endospore former might be harder to treat than one caused by a non-spore former?