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Biology LibreTexts

12.3: Results

  • Page ID
    16029
  • A. ELISA test

    Record the appearance of each of the wells here.

    Well Contents Color appearance Interpretation
    1
    2
    3
    4
    5
    6

    B. Biolog

    Record your Biolog results below.

    Describe the colony that you picked for Biolog (color, etc.): _________________________________________ ________________________________________________________________

    Use the sheet on the next page to record your results. In each box, write “+” if the well appears positive (purple color), “-” if there is no color change, and “+/-“ if the result is borderline (light purple).

    Your instructor will show you how to enter your results into the database to achieve an identification.

    An example of what a Biolog plate looks like is shown below.

    Identification: ___________________________________

    % match: _______________________________________

    biolog.png

    Figure 12.3.1: Biolog plate

    A1 Negative Control A2 Dextrin A3 D-Maltose A4 D-Trehalose A5 D-Cellobiose A6 Gentiobiose A7 Sucrose A8 D-Turanose A9 Stachyose A10 Positive Control A11 pH 6 A12 pH 5
    B1 D-Raffinose B2 α-D-Lactose B3 D-Melibiose B4 β-Methyl-D-Glucoside B5 D-Salicin B6 N-Acetyl-DGlucosamine B7 N-Acetyl-β-D-Mannosamine B8 N-Acetyl-D-Galactosamine B9 N-Acetyl Neuraminic Acid B10 1% NaCl B11 4% NaCl B12 8% NaCl
    C1 α-D-Glucose C2 D-Mannose C3 D-Fructose C4 D-Galactose C5 3-Methyl Glucose C6 D-Fucose C7 L-Fucose C8 L-Rhamnose C9 Inosine C10 1% Sodium Lactate C11 Fusidic Acid C12 D-Serine
    D1 D-Sorbitol D2 D-Mannitol D3 D-Arabitol D4 myo-Inositol D5 Glycerol D6 D-Glucose- 6- PO4 D7 D-Fructose- 6- PO4 D8 D-Aspartic Acid D9 D-Serine D10 Troleandomycin D11 Rifamycin SV D12 Minocycline
    E1 Gelatin E2 Glycyl-L-Proline E3 L-Alanine E4 L-Arginine E5 L-Aspartic Acid E6 L-Glutamic Acid E7 L-Histidine E8 L-Pyroglutamic Acid E9 L-Serine E10 Lincomycin E11 Guanidine HCl E12 Niaproof 4
    F1 Pectin Acid F2 D-Galacturonic Acid F3 L-Galactonic Acid Lactone F4 D-Gluconic Acid F5 D-Glucuronic Acid F6 Glucuronamide F7 Mucic Acid F8 Quinic Acid F9 D-Saccharic Acid F10 Vancomycin F11 Tetrazolium Violet F12 Tetrazolium Blue
    G1 p-Hydroxy Phenylacetic Acid G2 Methyl Pyruvate G3 D-Lactic Acid Methyl Ester G4 L-Lactic Acid G5 Citric Acid G6 α-KetoGlutaric Acid G7 D-Malic Acid G8 L-Malic Acid G9 BromoSuccinic Acid G10 Nalidixic Acid G11 Lithium Chloride G12 Potassium Tellurite
    H1 Tween 40 H2 γ-aminobutyric Acid H3 α- HydroxyButyric Acid H4 β-HydroxyD,LButyric Acid H5 α-Keto-Butyric Acid H6 Acetoacetic Acid H7 Propionic Acid H8 Acetic Acid H9 Formic Acid H10 Aztreonam H11 Sodium Butyrate H12 Sodium Bromate