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pH Gradient Leads to ATP Synthesis

How Does the pH Gradient Collapse Lead to ATP Synthesis?

The mechanism by which the proton gradient drives ATP synthesis involves a complex coupling of the F0 and F1 subunits. A more detailed image of the whole ATO complex is shown below.

Figure:  Detailed View of F0F1 ATP synthase structure

A04FoF1DetailStructure.gif

Closer views of the c subunits and a yellow rectangle representing the a subunit (missing in the combined crystal structure) are shown below; they comprise the Fo part of the complex. These subunits reside in the inner membrane of the mitochondria (or cell membrane of bacteria) and are involved in proton transport from the matrix (or cytoplasm of a bacteria) to the inner membrane space (or periplasmic space of bacteria). The multiple c subunits consist of two very hydrophobic helices connected by a loop in a helix-loop-helix motif.

A05csubunitATPSynth.GIF

Two classic inhibitors (structures shown below) of ATP synthase interact with the Fo subunit. One, oligomycin A, binds between the a and c subunits and blocks proton transport activity of the Fo subunit. Oligomycin A sensitivity requires, paradoxically, OSCP (Oligomycin-Sensitivity Conferring Protein which is analogous to the bacterial delta subunit), a stalk protein subunit distal to Fo which couples Fo and F1. Another inhibitor, dicyclohexylcarbodiimide, reacts with a protonated Asp 61 in c subunits of F0.  It does so even at pH 8.0, which indicates that the pKa of the Asp 61 is much higher than usual. This might occur if the Asp is a very hydrophobic environment. The modification of one Asp 61 in only one c subunit is necessary to stop Fo activity. The protonated carboxyl group donates a proton to a N atom in DCCD, which then reacts with the deprotonated Asp to form an O-acyl isourea derivative.

Figure: Structure of Oligomycin A and DCCD - Inhibitors of proton transport by Fo

A06DCCD_OligoA.gif

The figures below show the structure of the ac complex from E. coli. Protons flow to the a chain Arg 210 which is between two Asp 61 on adjacent c chains. One of the Asp 61 is protonated, allowing it to alter conformation and essentially ratchet in the membrane domain in a motion facilitated by the development of a neutral protonated Asp. 

A07R210_D61sInteract.jpg

Protons from the inner membrane space or in the periplasmic space (in the above figures) then flow from the periplasm by forming a "handshaking" proton transfer relay which delivers another proton to the deprotonated Arg 210, allowing the circular ratching of the c subunits in the membrane to continue. A set of polar residues entirely within subunit a, including Gln 252, Asn 214, Asn 148, Asp 119, His 245, Glu 219, Ser 144 and Asn 238, provide the path as illustrated below. 

A08HPathctoA_EColi_ATPSyn.jpg

 When a proton is passed to the unprotonated Asp 61, a conformational change in the protonated c subunit occurs. This leads to changes in c subunit interactions which seems to ratchet the c12 core. Since the c12 oligomer  contacts the g subunit connecting the Fo stalk and F1 ATPase units, the g subunit rotates, leading to sequential conformational changes in each of the 3 contacted  (ab)2 dimers of the F1 enzyme. This leads to changes in ATP affinity through cycling each through the L, O, and T conformations.

Figure:  Coupling Proton Flow in F0 to Conformation Change

A09FoF1totalB.gif

Reprinted by permission of Nature. Rastogi & Girvin.  Nature 402, 263-268 (1999) Copyright 1999 McMilllan Publishers LTD

  1.  Animation 1: ATP synthesis
  2.  Animation 2: ATP synthesis mechanism
  3.  F1F0 ATPase animation from Harvard
  4.  ATP sythase
  5.  Powering the Cell: Mitochondria
  6.  XVIVO: Scientific Animations
  7.  ATPase animations from the MRC
  8. General List of Movies

In summary, F0F1ATPase (or synthase) is a rotary enzyme that ultimately couples collapse of a proton gradient (a chemical potential gradient which contributes to the transmembrane electrical potential) to a chemical (phosphorylation) step. The rotor, which is in contact with both the F0 proton pore, and the F1 synthase, moves with respect to both subunits, which couples them.  Motion of course is relative so the rotor can be thought of as static with the F0 and F1 subunits as rotating. The F0 pore can hence be considered an electrical motor and the F1 synthase a chemical motor.  Carrying the analogy of a motor even further, the F0 electrical motor turns the F1 chemical motor into a generator, not of electricity but of ATP.  The figure and link below, taken from the Protein Data Bank, go into more depth about this nanomotor.

Figure: ATP Synthase Complex

25ATPsynthasePDB.gif